Entirely Automated Quantification of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in Plasma by Using the Ultrasensitive COBAS AMPLICOR HIV-1 Monitor Test and RNA Purification on the MagNA Pure LC Instrument
نویسندگان
چکیده
منابع مشابه
application of sybr-green real time rt-pcr for quantification of human immunodeficiency virus type-1 (hiv-1) and comparison with cobas amplicor test
objective: in this study, a sybr green real-time rt-pcr assay for quantification of hiv-1 viral rna was developed. materials and methods: this assay was performed based on amplification of the pol region of hiv-1 and product analysis by an abi 7500 system. we quantified hiv-1 viral load in 26 seropositive patients by this system and the data were subsequently compared with results obtained wit...
متن کاملStability of human immunodeficiency virus type 1 RNA in cerebrospinal fluid determined with the AMPLICOR HIV-1 MONITOR test, version 1.5 (ultrasensitive).
We evaluated the effects of time, temperature, freezing, and thawing on the cerebrospinal fluid viral load by using the Roche AMPLICOR HIV-1 MONITOR test, version 1.5 (ultrasensitive). While a statistically significant decrease from the baseline was observed at 24 h, but not at 6 or 12 h, and with one freeze-thaw cycle, all changes were within the range of intra-assay variability.
متن کاملNeed for procedural details in the protocol for specimen processing by the MagNA Pure LC Instrument.
We read with interest the favorable findings of Hölzl et al. (1) showing good agreement of plasma HIV-1 RNA measurements (by the ultrasensitive COBAS AMPLICOR HIV-1 Monitor test) by using the conventional (manual) RNA purification method and the fully automated method performed with the MagNA Pure LC instrument. Their findings would enable diagnostic laboratories to use a commercially available...
متن کاملPerformance evaluation of the new Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version 2.0 for quantification of human immunodeficiency virus type 1 RNA.
Despite FDA approval and CE marking of commercial tests, manufacturer-independent testing of the technical aspects of newly developed tests is important. To evaluate the analytical performance and explore the clinical applicability of the new Roche COBAS AmpliPrep COBAS TaqMan HIV-1 test, version 2.0 (CAP/CTM v2.0), platform comparison was performed with the Roche CAP/CTM test, version 2.0, the...
متن کاملQuantification of genital human immunodeficiency virus type 1 (HIV-1) DNA in specimens from women with low plasma HIV-1 RNA levels typical of HIV-1 nontransmitters.
Studies of human immunodeficiency virus type 1 (HIV-1) transmission suggest that genital HIV-1 RNA and DNA may both be determinants of HIV-1 infectivity. Despite its potential role in HIV-1 transmission, there are limited quantitative data on genital HIV-1 DNA. Here we validated an in-house real-time PCR method for quantification of HIV-1 DNA in genital specimens. In reactions with 100 genomes ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2003
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.41.3.1248-1251.2003